FIG. 2.

Effects of MOI and inactivation of nonadsorbed virus on the proportion of viral DNA associated with histone H3. ChIP was carried out at 3 hpi with an antibody for histone H3, and real-time time PCR was undertaken using primers for the region downstream of the ICP4 transcriptional start site and analyzed as for Fig. 1. (A) HeLa cells were infected at MOIs between 0.1 and 5 PFU/cell. (B) HeLa cells were infected at an MOI of 0.1 PFU/cell. After a 1-h adsorption period, the medium was replaced without washing the cells, or the cells were washed with PBS alone or washed with PBS followed by a low-pH wash buffer. The means and standard errors of the means of at least four independent experiments are shown.