FIG. 6.

Kinetics of histone H3 association with the ICP4 and ICP8 genes following infection with a ICP0⁻ virus. HeLa cells were infected at an MOI of 0.1 PFU/cell with either an ICP0⁻ virus (7134) (B and D) or rescued virus (7134R) (A and C). Cell lysates were prepared at 3 and 8 hpi. The fraction of ICP4 downstream (A and B) or ICP8 downstream (C and D) DNA immunoprecipitated determined by real-time PCR was normalized to the fraction of GAPDH DNA immunoprecipitated. Lines connect values for individual experiments. Samples with mean values that varied significantly (P < 0.05, paired Student's t test) are indicated (*).