TABLE 1.
Primers used for real-time PCR analysis
| DNA targeta | Sequence | Locatione |
|---|---|---|
| ICP4 USa | 5′-CGCATGGCATCTCATTACCG-3′ (forward) | −293 to −256 |
| 5′-TAGCATGCGGAACGGAAGC-3′ (reverse) | ||
| ICP4 SSb | 5′-GCGCTCCGTGTGGACGAT-3′ (forward) | −23 to +30 |
| 5′-CGGCCCCTGGGACTATATGA-3′ (reverse) | ||
| ICP4 DS | 5′-GCCCGGGCGCTGCTTGTTCTCC-3′ (forward) | +265 to +332 |
| 5′-CGTCCGCCGTCGCAGCCGTATC-3′ (reverse) | ||
| ICP8 SSb | 5′-CCACGCCCACCGGCTGATGAC-3′ (forward) | −100 to +28 |
| 5′-TGCTTACGGTCAGGTGCTCCG-3′ (reverse) | ||
| ICP8 DSc | 5′-GAGACCGGGGTTGGGGAATGAATC-3′ (forward) | +95 to +144 |
| 5′-CCCCGGGGGTTGTCTGTGAAGG-3′ (reverse) | ||
| UL44 USb | 5′-CCGACCGCCCGCCCGTTGAC-3′ (forward) | −225 to −125 |
| 5′-ACGCTTCGGGGCCTCTTCTTCTCC-3′ (reverse) | ||
| UL44 SSb | 5′-GGGTATAAATTCCGGAAGGGG-3′ (forward) | −30 to +130 |
| 5′-CTGCGAGGGATCGGCTAGCG-3′ (reverse) | ||
| GAPDHd | 5′-TTCGACAGTCAGCCGCATCTTCTT-3′ (forward) | +44 to +153 |
| 5′-CAGGCGCCCAATACGACCAAATC-3′ (reverse) |
a
US, upstream; DS, downstream; SS, start site.
b
Primer sequences were taken from the HSV-1 sequence available in GenBank (accession no. NC_001806).
c
Primer sequences were taken from the KOS sequence kindly provided by Robert Colgrove.
d
Primer sequences were taken from the GAPDH sequence available in GenBank (accession no. NC_002046).
e
Relative to the transcriptional start site.