TABLE 1.
Kinetic parameters for the mechanism of FMDV and PV 3Dpol stability and 3Dpol primer-template complex assembly/stabilitya
| Virus 3Dpol | Rate of assembly (s−1) | Rate of dissociation for:
|
Inactivation at:
|
|||
|---|---|---|---|---|---|---|
| ERn (10−5 s−1) | ERn + 1 (10−5 s−1) | 4°C (10−5 s−1) | 22°C (10−5 s−1) | 30°C (10−3 s−1) | ||
| FMDV | 0.003 | 9 | 0.7 | 0.6c | 1c | 3 |
| PVb | 0.08 | 60 | 4 | 20 | 500 | 20 |
| Change (n-fold) | 30 | 7 | 6 | 30 | 500 | 7 |
a
All values are rounded to one significant figure. The error in each measurement is no greater than 20%, unless indicated otherwise. Experiments were performed as described in Materials and Methods.
b
Values for PV are taken from reference 6.
c
The error in the measurement was 50%.