FIG. 6.

Brd4 is preferentially localized to the FR element of the EBV genome. ChIP experiments were performed on EBV-positive Raji cells using antibodies against Brd4, EBNA1, or nonspecific rabbit IgG as a negative control. Recovered DNA fragments were quantified by real-time PCR using primer sets for the oriP DS and FR elements and the BZLF1 promoter region. The amplification signals were normalized to those from the same cell lysates prior to IP using the same primer pairs. Signals from Brd4 and EBNA1 antibody samples are expressed relative to the control IgG sample, which was set to 1. The results shown are from three independent experiments with PCR quantification performed in triplicate for each experiment. For Brd4 samples, only the FR fragment result is statistically significantly different from the IgG control, with a P value of 0.000007.