Growth curves of YFVs in BHK cells. Experiments were conducted as described in Materials and Methods, using a multiplicity of infection of 0.001. The values are mean log PFU per milliliter ± 1 standard deviation for triplicate samples. (A) SPYF-H3, E326-E, E305-V, E380-T, and YF5.2iv viruses. (B) Viruses containing substitutions at position 326.