TABLE 2.
VHH kinetic constants and affinities for IIIB gp120a
| VHH | KD (nM) | ka [1/(M·s)] | kD (1/s) |
|---|---|---|---|
| A12 | 0.1 | 2.73 × 105 | 2.98 × 10−5 |
| D7 | 0.097 | 4.00 × 105 | 3.89 × 10−5 |
| C8 | 0.85 | 2.05 × 105 | 1.74 × 10−4 |
VHH A12, D7, and C8 association rate constants (ka), dissociation rate constants (kD), and equilibrium dissociation constants (KD = kD/ka) for recombinant IIIB gp120 were determined in surface plasmon resonance studies.