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. 2008 Oct 1;82(24):12406–12415. doi: 10.1128/JVI.01605-08

FIG. 2.

FIG. 2.

Expression of AncuEPV fusolin gene using a baculovirus expression system. (A) Western blot analysis of Sf21 cells infected with a baculovirus-based vector harboring a His-tagged AncuEPV fusolin gene. Lane 1, native fusolin derived from AncuEPV spindles (positive control); lane 2, whole-cell culture; lane 3, conditioned medium (secreted fraction); lane 4, soluble protein fraction; lane 5, insoluble protein fraction; lane M, molecular mass standard. The position of the fusolin visualized by alkaline phosphatase-BCIP-NBT staining is indicated by an arrow. (B) SDS-PAGE analysis of the recombinant fusolin purified from vector-infected cells. Lane 1, recombinant AncuEPV fusolin; lane 2, His-tagged β-glucuronidase. The position of the fusolin stained with Coomassie brilliant blue is indicated by an arrow.