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Glycosylation of the recombinant fusolin. Recombinant proteins were subjected to SDS-PAGE and electroblotted onto a PVDF membrane. Blotted membranes were stained with Coomassie brilliant blue (CBB) (left) or PAS (right). Spindle, native fusolin derived from AncuEPV spindles; rFusolin, recombinant fusolin; M, molecular mass standard. BSA was used as a negative control. The position of the fusolin is indicated by an arrow.
