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. 2008 Oct 1;82(24):12406–12415. doi: 10.1128/JVI.01605-08

FIG. 4.

FIG. 4.

Proteolytic processing of fusolin in insect digestive juice. (A) Western blot analysis of the recombinant fusolin treated with digestive juice harvested from B. mori larvae. The recombinant fusolins were analyzed before (lane 1) and after (lane 2) treatment with the digestive juice. Closed and open arrowheads indicate positions of full-length (49-kDa) and processed (35-kDa) fusolins, respectively. Lane M, molecular mass standard. (B) Inhibition assay of the C-terminal degradation of fusolin. Lane 1, recombinant fusolin (untreated); lane 2, recombinant fusolin treated with digestive juice; lanes 3 to 6, recombinant fusolin treated with digestive juice containing protease inhibitors (Pefabloc SC Plus [serine protease inhibitor]) (lane 3), E64 (cysteine protease inhibitor) (lane 4), pepstatin A (aspartic protease inhibitor) (lane 5), and EDTA (metalloprotease inhibitor) (lane 6); lane M, molecular mass standard. Closed and open arrowheads indicate the same as described for panel A.