TABLE 4.
Inoculum | No. of infected larvae/no. of tested larvaeb | Infection rate (%) |
---|---|---|
AncuEPV spheroids alone | 5/32 | 15.6 |
AncuEPV spheroids combined with rFusolin | 23/33* | 69.7 |
AncuEPV spheroids combined with rGUS | 0/18 | 0 |
rFusolin | 0/30 | 0 |
Sterile water | 0/29 | 0 |
Second-instar first-day larvae of A. cuprea were used in this test. A total of 1.5×103 of AncuEPV spheroids was inoculated per larva, and 9 μg of rFusolin or rGUS was administrated per larva as an additive.
*, significant difference (P < 0.01, chi-square test) in the ratio of infected to uninfected larvae compared to inoculation of the spheroids alone.