TABLE 2.
Cell type and day p.i. | Total no. of cells (SE)
|
||
---|---|---|---|
WT | MM | PBS | |
CD4+ | |||
2 | 1.5 × 103 (3.0) | 3.1 × 103 (2.2) | 2.5 × 103 (1.5) |
4 | 16.5 × 103 (3.1) | 18.5 × 103 (2.5) | 14.4 × 103 (2.8) |
6 | 16.2 × 103 (2.1) | 16.0 × 103 (2.4) | 12.2 × 103 (1.6) |
8 | 33.5 × 103 (5.8) | 29.5 × 103 (6.0) | 31.0 × 103 (5.2) |
CD8+ | |||
2 | 2.8 × 103 (1.1) | 5.8 × 103 (2.2) | 5.1 × 103 (1.5) |
4 | 18.5 × 103 (1.8) | 17.9 × 103 (2.8) | 18.9 × 103 (2.8) |
6 | 18.4 × 103 (2.5) | 21.0 × 103 (2.1) | 19.4 × 103 (2.6) |
8 | 48.6 × 103 (5.5) | 39.5 × 103 (5.7) | 48.0 × 103 (5.5) |
B220+ | |||
2 | 0.09 × 103 (0.1) | 0.1 × 103 (0.1) | 0.1 × 103 (0.1) |
4 | 0.5 × 103 (0.1) | 0.2 × 103 (0.1) | 0.8 × 103 (0.1) |
6 | 0.5 × 103 (0.5) | 0.6 × 103 (0.2) | 0.5 × 103 (0.1) |
8 | 0.3 × 103 (0.1) | 0.1 × 103 (0.1) | 0.6 × 103 (0.2) |
DX5+ | |||
2 | 2.5 × 103 (0.5)† | 5.0 × 103 (1.1) | 4.8 × 103 (1.2) |
4 | 5.0 × 103 (0.5) | 8.0 × 103 (1.2) | 6.5 × 103 (1.3) |
6 | 7.5 × 103 (1.2)†‡ | 14.4 × 103 (2.8)‡ | 1.3 × 103 (1.1)† |
8 | 1.9 × 103 (1.1)† | 7.0 × 103 (1.2) | 0.5 × 103 (0.5)† |
RB6-8C5+ | |||
2 | 3.8 × 103 (0.8)†‡ | 11.8 × 103 (1.8)* | 10.0 × 103 (1.5)* |
4 | 1.9 × 103 (0.8) | 2.5 × 103 (1.8) | 3.3 × 103 (2.5) |
6 | 5.8 × 103 (2.4) | 6.0 × 103 (1.8) | 7.4 × 103 (2.0) |
8 | 8.8 × 103 (1.4) | 8.0 × 103 (2.7) | 9.8 × 103 (0.4) |
CD11b+ | |||
2 | 3.1 × 103 (0.5)†‡ | 5.9 × 103 (0.5) | 5.1 × 103 (0.5) |
4 | 10.0 × 103 (2.8) | 10.8 × 103 (2.8) | 11.0 × 103 (2.2) |
6 | 5.0 × 103 (1.2) | 4.1 × 103 (1.5) | 5.9 × 103 (1.5) |
8 | 5.5 × 103 (1.8)† | 9.0 × 103 (1.1) | 7.5 × 103 (1.5) |
Mice were i.n. treated with 100 nM (2 mg/kg) WT siRNA specific for the RSV P gene, 100 nM (2 mg/kg) MM siRNA, or PBS prior to RSV/A2 infection (106 PFU). The total cell numbers were determined from four to five mice/treatment in three separate experiments using appropriate monoclonal antibodies and flow cytometry. *, Statistically different (P < 0.05) from WT-treated mice; †, statistically different (P < 0.05) from MM-treated mice; ‡, statistically different (P < 0.05) from PBS-treated mice.