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Internal lysines and GADD34 degradation. (A) C-terminal HA-tagged wild-type (WT) and lysineless (0K) GADD34 polypeptides, residues 1 to 155, were expressed in HEK293T cells and subjected to turnover analyses in the absence or presence of 5 μM MG262. Lysates were generated at 0, 1.5, and 3 h following the addition of CHX; subjected to SDS-PAGE; and immunoblotted for HA and tubulin.
