A-D.) Immunohistochemical co-localization of TRPV1 (blue), AKAP220 (red) and PKA RII (green) in cultured TG neurons from rat, scale bar in yellow represents 50 μm.
E.) Western blot analysis of AKAP220 and AKAP150 expression from 50 μg TG neuronal lysate following siRNA knock-down: lane 1, no transfection; lane 2, mock; lane 3, AKAP150-1 FITC siRNA; lane 4, AKAP220-1 siRNA; lane 5, AKAP220-2 siRNA.
F.) Cultured TG neurons mock transfected (Mock) or transfected with AKAP220-1 siRNA (AKAP220 siRNA) and analyzed for ICAP (50 nm) following vehicle (H20) or 8-Br-cAMP (10 μM) treatment. n=7-12/group.
G-H.) Representative trace of recordings taken from cultured TG neurons transfected with mock (G) or AKAP220 (H) siRNA, treatments indicated.
All plotted data are expressed as mean ± SE, **p<0.01, ***p<0.005.