Fig. 2.

The activity of nHDL requires apoA-II but not apoA-I, apoC-I, apoC-III, or apoE. THP-1 cells were stimulated with LPS as described in the caption to Figure 1 in the presence of an inhibitory concentration of LBP and 100 μg/ml of nHDL isolated from humans, wild-type mice, or apolipoprotein gene knock-out mice as indicated. TNF production is expressed as the fold change from the control (no HDL). Error bars denote mean ± SEM of duplicate determinations in 2-9 experiments (n = 4-18). All HDLs were significantly different from the control (P < 0.0001) except for apoA-II knock-out HDL (P = 0.2). Wild-type HDL was significantly different from apoA-II knock-out HDL (P = 0.0004), apoC-I/C-III/E knock-out HDL (P = 0.0013), and human HDL (P = 0.0019).