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. 2008 Oct 20;28(24):7402–7413. doi: 10.1128/MCB.01108-08

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Copyright © 2008, American Society for Microbiology

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FIG. 1. — Expression and subcellular localization of mCherry-tagged DDB1. (A) Immunoblot analysis of parental VH10SV40 cells and VH10SV40 cells stably expressing mCherry-DDB1 (A634), using antibodies against DDB1. (B) Subcellular localization of mCherry-DDB1 in A634 cells. (C) Subcellular localization of endogenous DDB1 in cells detected by immunostaining of VH10SV40 cells by use of antibodies against DDB1. (D) Subcellular localization of mCherry-DDB1 in VH10SV40 cells transiently transfected with the mCherry-DDB1-expressing construct (48 h after transfection). (E) Nuclear localization of mCherry-DDB1 in VH10SV40 cells transiently cotransfected with mCherry-DDB1- and eYFP-DDB2-expressing constructs (48 h after transfection).