FIG. 2.
Recruitment of mCherry-DDB1 to UV-damaged DNA. (A) Accumulation of mCherry-DDB1 at the site of local damage in A634 cells (indicated with an arrow) irradiated with 100 J/m2 of UV-C through a polycarbonate filter (5 min after UV irradiation). (B) Quantification of the accumulation kinetics of mCherry-DDB1, eYFP-DDB2, and GFP-CUL4A at the site of local damage. Curves were normalized to the plateau value. Time point 0 corresponds to the beginning of the UV irradiation. (C) FRAP analysis of mCherry-DDB1 mobility in mock-irradiated (M.I.) and UV-irradiated (10 J/m2) A634 cells. Experimental curves, curves obtained by Monte Carlo simulations (sim.), and residuals (res.) are shown.