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. 2008 Oct 13;28(24):7427–7441. doi: 10.1128/MCB.01962-07

FIG. 6.

FIG. 6.

Physical and functional interactions among N1IC, β-catenin, p300, and P/CAF in hes1 promoter activation. (A and B) HEK293 cells were transfected with expression vectors containing tagged cDNAs. The molecular interaction between N1IC and P/CAF was facilitated in the presence of both p300 and β-catenin (βcat). Furthermore, β-catenin was efficiently immunoprecipitated with N1IC in the presence of both p300 and P/CAF (B). IP; αCont IgG, immunoprecipitation with anti-control IgG. (C) The contribution of endogenous p300 to hes1 promoter activation in neural precursor cells was examined using truncated forms of dominant-negative p300 (n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001; Student's t test). stb, stabilized. (D) Neural precursor cells were transfected with combinations of pHes1-Luc, N1IC, stabilized β-catenin, and P/CAF and p300 cDNAs (n = 4; *, P < 0.05; **, P < 0.01; Student's t test). αmyc, anti-myc antibody.