TABLE 3.

Genes associated with ERα binding sites in only MCF-7AKT cells and are differentially expressed in two cell types^a

GenBank no.	Gene symbol	Strand	No. of binding sites in MCF-7AKT+E2	Fold change upon E2		Differential expression in MCF-7p and MCF-7AKT	P
				MCF-7p	MCF-7AKT
NM_005185	CALML3	+	1	1.10	0.93*	0.84	2.2E-02
NM_005197	FOXN3	−	2	1.03	0.90*	0.88	4.3E-02
NM_000196	HSD11B2	+	2	1.36*	1.63*	1.19	1.1E-02
NM_016095	GINS2	−	1	1.46*	1.70*	1.17	4.3E-02
NM_002274	KRT13	−	2	1.48*	1.91*	1.28	3.9E-02
NM_016077	PTRH2	−	1	1.68*	1.87*	1.12	2.4E-02
NM_006640	SINT1	+	1	1.45*	1.71*	1.18	4.7E-02
NM_004036	ADCY3	−	1	1.49*	1.71*	1.15	4.7E-02
NM_015341	NCAPH	+	1	1.08*	0.94	0.87	2.6E-02
NM_018288	PHF10	−	1	1.01	1.18*	1.17	3.1E-03
NM_019848	SLC10A3	−	1	1.34*	1.67*	1.24	1.3E-03
NM_001066	TNFRSF1B	+	1	0.94*	1.09	1.16	9.6E-03
NM_030952	NUAK2	−	1	0.79*	0.92*	1.16	1.2E-02
NM_173576	MKX	−	1	0.70*	0.55*	0.78	1.3E-02
NM_080760	DACH1	−	2	0.90*	1.05	1.17	8.6E-03
NM_005358	LMO7	+	1	0.74*	0.86*	1.17	4.5E-02
NM_032714	C14orf151	+	1	0.83*	0.98	1.18	4.8E-02
NM_016084	RASD1	−	1	0.45*	0.72*	1.60	8.5E-03
NM_178233	OTOP3	+	1	0.92*	1.05	1.14	3.1E-02
NM_019850	NGEF	−	1	0.93*	1.04*	1.12	1.7E-02
NM_005668	ST8SIA4	−	1	0.63*	0.47*	0.74	3.9E-03

^aDifferential expression in MCF-7p and MCF-7AKT cells of >1: estrogen-induced gene upregulation is greater in MCF-7AKT cells than in MCF-7p cells or estrogen-mediated downregulation is greater in MCF-7p cells than in MCF-7AKT cells. Differential expression in MCF-7p and MCF-7AKT cells of <1: estrogen-induced gene upregulation is greater in MCF-7p cells than in MCF-7AKT cells or estrogen-mediated downregulation is greater in MCF-7AKT cells than in MCF-7p cells. *, genes whose expression was significantly different in untreated and estrogen-treated cells.