FIG. 6.

ChIP analysis of the E2f2 promoter region in sorted R1/R2 E13.5 fetal liver cells from wild-type embryos expressing HA-EKLF. Chromatin fragments were precipitated with anti-HA antibody and amplified with six sets of primers from the E2f2 promoter region. Regions 1 to 5 contain one or more consensus EKLF binding motifs (NCNCNCCCN). The negative control region (-C) does not contain an EKLF binding motif. The location of the primers flanking each region is indicated. The dark gray bars represent the enrichment of each sequence relative to the -C region (gray bar; designated as 1.0).