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Direct PCRs to detect alternative splicing of human GTPBP3 exon 8A. Primers used are represented by arrows on the diagrams above the gels. PCR analysis was performed on cDNAs from eight different human tissues (Human I MTC panel; Clontech, California) and cDNA synthesized on DNase-treated mRNA isolated from human fibroblasts and lymphocytes and HEK-293 cells. M1, GeneRuler 100-bp DNA Ladder (Fermentas); M2, pUC19 DNA/MspI Marker, 23 (Fermentas).
