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Direct PCRs to detect mRNA variants of human GTPBP3 that include exon 9B but differ in the splicing of exon 8A. Primers used are represented by arrows on the diagram and are indicated over each gel. Primer CNT1 spans the junction region between exons 7 and 8A, whereas CNT33 spans that between exons 7 and 8B. PCR analysis was performed on cDNAs from the indicated human tissues (Human I MTC panel; Clontech, California). M, GeneRuler 100-bp DNA Ladder (Fermentas).
