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. 2008 Oct 13;28(24):7514–7531. doi: 10.1128/MCB.00946-08

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FIG. 7. — Binding of GST-GTPBP3 proteins to mGDP and AlF_x. Fluorescence spectra (excited at 360 nm) include GTPase buffer (a) and the same solution after subsequent addition of 2 μM mGDP only (b), 10 μM protein (c), 10 mM NaF (d), and 70 μM AlCl₃ (e). The table underneath the figure summarizes the conditions under which the spectra were obtained. Note that AlF_x is formed by mixing an aluminum salt (AlCl₃) with NaF. Fluorescence emission spectra were recorded 15 min after AlCl₃ addition. λ, wavelength; I_F, fluorescence intensity; au, arbitrary units. (A) MnmE. (B) GST-MnmE. (C) GST-GTPBP3(Ins8A). (D) GST-GTPBP3(Del8A). (E) GST-GTPBP3(Ins8A) (KCl was replaced by NaCl in the assay buffer). (F) GST-GTPBP3(Del8A) (KCl was replaced by NaCl in the assay buffer).