TABLE 1.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pBAD22 | Expression vector with PB and AraC control (Apr) | 13 |
| pCR-XL-TOPO | Cloning vector (Kanr) | Invitrogen |
| pCR3.1 | Eukaryotic expression vector with Pcmv control (Apr Kanr Neor) | Invitrogen |
| pEGFP-N1 | Eukaryotic expression vector for GFP fusions (Kanr Neor) | Clontech |
| pET15b | His tag fusion vector (Apr) | Novagen |
| pGEX4T1 | GST fusion vector (Apr) | GE Healthcare |
| pIC684 | GST-MnmE fusion | 6 |
| pIC982 | GTPBP3(Del8A) cloned into pCR-XL-TOPO | This work |
| pIC985 | GTPBP3(Ins8A) cloned into pCR-XL-TOPO | This work |
| pIC986 | GTPBP3(Del8A) inserted into pET15b digested with NdeI and BamHI | This work |
| pIC988 | GTPBP3(Ins8A) inserted into pET15b digested with NdeI and BamHI | This work |
| pIC998 | GTPBP3(Ins8A) inserted into EcoRI-cut, filled-in pEGFP-N1 | This work |
| pIC1026 | GTPBP3(Del8A) inserted into EcoRI-cut, filled-in pEGFP-N1 | This work |
| pIC1048 | pIC998 derivative lacking GFP | This work |
| pIC1055 | BglII-XbaI fragment of pIC1048 inserted between the BamHI and XbaI sites on pCR3.1 | This work |
| pIC1089 | pIC1298-related plasmid containing mutation G261A | This work |
| pIC1296 | FLAG-(ΔN)GTPBP3 inserted between sites NcoI and SalI on pBAD22 | This work |
| pIC1298 | GST fusion of GTPBP3(Ins8A) | This work |
| pIC1299 | GST fusion of GTPBP3(Del8A) | This work |
| pIC1318 | GTPBP3 G-domain inserted between sites NcoI and XhoI on pBAD22 | This work |
| pIC1376 | GTPBP3 N-terminal domain inserted between sites NcoI and XhoI on pET15b | This work |