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Binding parameters of MnmE and GTPBP3 proteins
| Protein |
Kd (μM)
|
|
|---|---|---|
| mGTPγS | mGDP | |
| MnmE | 1.29 ± 0.15 | 3.19 ± 0.23 |
| GST-MnmE | 1.08 ± 0.06 | 2.21 ± 0.22 |
| GST-GTPBP3(Ins8A) | 0.55 ± 0.05 | 1.04 ± 0.05 |
| GST-GTPBP8(Del8A) | 0.41 ± 0.05 | 1.06 ± 0.01 |
| FLAG-(ΔN)GTPBP3 | 0.64 ± 0.06 | 1.71 ± 0.09 |
| GTPBP3 G domain | 0.70 ± 0.07 | 2.17 ± 0.13 |
