TABLE 4.
GTPase activities of MnmE and GTPBP3 proteins
| Protein | kcat/min |
|---|---|
| MnmE | 11.645 ± 0.145 |
| GST-MnmE | 10.834 ± 0.262 |
| GST-GTPBP3(Ins8A)a | 0.076 ± 0.008 |
| GST-GTPBP3(Del8A)a | 0.091 ± 0.001 |
| GST-GTPBP3(Ins8A) G261Aa,b | 0.016 ± 0.001 |
| FLAG-(ΔN)GTPBP3a | 0.070 ± 0.007 |
| GTPBP3 G domain | 0.065 ± 0.005 |
a
The Km for GTP hydrolysis by GTPBP3 proteins (calculated as specified in Materials in Methods) was around 250 μM.
b
GST-GTPBP3(Ins8A) G261A is a mutant protein impaired for guanine nucleotide binding, which was used as a negative control in these experiments (for location of residue G261, see Fig. 2).