FIG. 2.

ChIP scanning analysis of the c-Myc genomic locus in HCT116 cells. (A) Red boxes correspond to the amplicons produced in PCRs using oligonucleotide primer sets designed across the c-Myc genomic locus. These amplicons are arbitrarily designated 1 to 16. Purple and green vertical lines indicate the positions of consensus TCF motifs and β-catenin SACO GSTs, respectively. c-Myc is depicted at the top of the diagram with an arrow marking the transcription start site. (B) Real-time PCR analysis of ChIP assays performed in HCT116 cells using antibodies directed against β-catenin (red bars), TCF4 (blue bars), or control β-galactosidase (gray bars). The amplicons produced in the real-time PCRs are labeled 1 to 16 along the x axis. Real-time PCR analysis of ChIP assays in HCT116 cells using anti-TBP (C), anti-RNAPII S5 (D), anti-RNAPII (E), or anti-H3K9/K14ac and anti-H3K4me3 (F) antibodies. Error bars indicate standard errors of the means.