Fig. 3.

Effects of culture on selected proteins involved in excitation-contraction coupling. Myocyte homogenates were prepared from day 0 WT (WT-D0), day 0 PLM KO (KO-D0), day 2 PLM KO (KO-D2), day 2 GFP-expressing KO (KO-GFP), and day 2 PLM-expressing KO (KO-PLM) myocytes and subjected to SDS-PAGE followed by Western blot analysis. Primary antibodies (methods) were used to detect cardiac Na⁺/Ca²⁺ exchanger (NCX1), the α₁-subunit of Na⁺-K⁺-ATPase, sarco(endo)plasmic reticulum Ca²⁺-ATPase 2 (SERCA2), and calsequestrin (CLSQ). Composite results are shown in Table 1.