Fig. 1.

Engineering of a responder transgene construct. A schematic of the tetracycline (tet)-responsive α-myosin heavy chain (MHC) promoter construct expressing iSH2-p110α is shown. The full-length α-MHC promoter has 3 GATA sites and 2 thyroid response elements deleted with other cardiac-specific cis-acting elements intact. There are 7 repeats of the tet operator (tetO) sequences adjacent to the TATA box. The human growth hormone (hGH) poly adenylation (polyA) signal was immediately downstream of iSH2-p110α.