Fig. 4.
Temporal overexpression of cardiac-specific PI3Kα induces upregulation of Ca2+-regulating proteins. Three-month-old male double transgenic littermates were maintained with Dox water (control; n = 4) or regular drinking water (PI3Kα; n = 3) for 2 wk. Western blotting was performed using antibodies against L-type voltage-gated Ca2+ channel (Cav1.2), ryanodine receptor (RyR) type 2, and sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a; top). Actin levels were used as the loading control. Bottom: results of densitometric measurement of each antibody from 3 separate experiments. *P < 0.05 vs. control.