Figure 2.

Pertussis toxin inhibits neutrophil clearance by the spleen and bone marrow. Purified, senescent neutrophils labeled with ¹¹¹In were incubated for 1 h with 1 μg/ml pertussis toxin or PBS and subsequently administered i.v. into recipient mice. Organs were harvested after 2 h, and the radioactivity in each tissue was calculated as a percentage of the total present in the whole animal. Data are presented as mean ± se; n = 3 experiments, 3 mice/group. ***P ≤ 0.001; 2-way ANOVA and Bonferroni’s posttest.