Figure 5.

Neutralization of IL-17 does not significantly alter circulating neutrophil numbers in naive mice. A) Anti-G-CSF (7.5 mg/kg), anti-IL-17 or control IgG (10 mg/kg) was administered to naive mice at days 0 and 2 (anti-IL-17 only). The percentage of circulating neutrophils was determined by differential cell counts on the days indicated. Data are presented as mean ± se; n = 4–10. **P ≤ 0.01; 2-way ANOVA and Bonferroni’s posttest. B) To demonstrate the effectiveness of the anti-IL-17 in vivo, neutrophil recruitment to the peritoneum was assessed 4 h after the i.p. injection of 0.5 μg/mouse rIL-17. Neutrophil numbers were determined by differential cell counts. Data are presented as mean ± se, n = 4. **P ≤ 0.01; 1-way ANOVA and Dunnet’s posttest.