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letter
. 2008 Dec;18(12):1938–1943. doi: 10.1101/gr.078808.108

Figure 3.

Figure 3.

Pachytene FISH mapping of the MSY and the centromere of the Yh chromosome. (a) FISH mapping of seven BAC clones. BACs 69A15 and 86B15, which mark the boundary of MSY, were mapped outside of knobs K1 and K5, respectively. FISH signals pointed to by arrowheads 1–5 were derived from BACs 12I03, 99O03, 95B12, 52H15, and 85B24, respectively. Bar, 10 μm. (b) The same pachytene cell image was converted to a black-white image. The locations of the FISH signals derived from the five BACs matched the locations of the five knobs. (c) FISH mapping of K4-specific BAC 52H15 (red) and K5-specific 85B24 (green) on metaphase I (MI) XYh bivalent chromosome. K4 is positioned more poleward than K5. (d) MI FISH mapping of K4-specific 52H15 (green) and K3-specific BAC 47B08 (red). K4 is positioned more poleward than K3. (e) MI FISH mapping of K3-specific BAC 47B08 (red) and K2-specific 99O03 (green). (f) MI FISH mapping of K2-specific 99O03 (red) and K1-specific BAC 12I03 (green). The white arrow points to the K1 on the X chromosome.