Figure 3.

MITF-regulated miRNAs. (A) MITF occupancy (blue down arrow) of an E-box-containing nucleosome-depleted region upstream of miR-146a transcription initiation region (red right-pointing arrow) was shown by ChIP (Supplemental Material) in MALME and UACC62. (B,C) Both pri-miR-146a (B) and mature miR-146a (C) expression levels are increased and decreased by MITF overexpression and down-regulation, respectively, using adenoviruses expressing wild-type (wt) and naturally occurring dominant-negative (dn) MITF (Du et al. 2004) and siRNA against MITF in UACC62. Data presented are mean ± SEM from three independent experiments.