Figure 5.

RNAPIII-occupied miRNAs. (A) α-Amanitin sensitivities of RNAPII-transcribed miR-146a. The level of pri-miR-146a goes down quickly at a low dose of α-amanitin, supporting that it is transcribed by RNAPII and not by RNAPIII. (B) RNAPIII occupancy was observed in proximity to miR-565 transcription initation region. (C,D) α-Amanitin sensitivities of RNAPIII-transcribed miR-565 (C) and RNAPII- and RNAPIII-occupied mir-148a (D) were measured. The Y-axis shows fold change relative to the control sample, normalized to 18S rRNA. Data presented are means ± SEM from three independent experiments.