Fig. 4.

Enhanced gene expression resulting from interchromosomal interactions and the requirement for a key nuclear motor component. (A) Effects of nuclear microinjection of siRNA or antibody against DLCI on inhibiting E₂-induced TFF1:GREB1 interactions (**, P < 0.001 by χ²). (B–D) Cells were treated with siRNAs against DLCI and/or BAF53, and the expression of specific genes as indicated, were quantified by RT-qPCR. Mean ± SD of triplicate determinations. (E) RNA FISH demonstrates the requirement for interchromosomal interactions to achieve enhanced, E₂-induced gene expression. Quantification of expression from noninteracting (NI) and interacting allelic regions was based on the diameter of individual signals, which was then converted to volume (M ± SEM). Plot shows the significant increase of expression even in the absence of colocalization of either allele; and the comparison of interacting: noninteracting alleles (*, P < 0.01; **, P < 0.001 by t test).