Figure 3. SEB-exposed CD4+Vβ8+ T cells remain anergic after cell sorting.
Sorted CD4+Vβ8+ splenic T cells from BALB/c mice that had been injected 7 d previously with SEB (black bars) or PBS (hatched bars) were rested for 3 days in media + IL-7 then subjected to a [3H]-thymidine proliferation assay. Low [3H]-thymidine incorporation reflects lack of proliferation in response to restimulation with SEB, indicating anergy. Results from restimulation with SEA reflect the absence of Vβ3+ T cells in the sorted population.