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. Author manuscript; available in PMC: 2008 Dec 3.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1439–1448. doi: 10.1038/sj.onc.1210770

Figure 4. Src or p130Cas shRNA knockdown inhibits α4β 1 NB cell motility whereas anti-FAK shRNA selectively inhibits α5β 1 NB cell migration.

Figure 4

(a) Anti-FAK, p130Cas, paxillin, and Src immunoblots of NB cell lysates stably expressing scrambled or the indicated shRNAs. Actin blotting verified equal loading. (b) Phase contrast images of NB8 parental or the indicated shRNA-expressing cells growing in culture. Scale bar is 20 μm. (c) Expression levels of Pyk2 (left panel) or Hic5 (right panel) in NB8 parental or FAK-paxillin shRNA expressing cells. 293T cell lysates were used for Pyk2 positive or Hic5 negative expression control. NB8 cell haptotaxis motility on FN-(CS-1) (d)* and FN-(9-11) (e). *See Fig.1.