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. Author manuscript; available in PMC: 2008 Dec 3.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1439–1448. doi: 10.1038/sj.onc.1210770

Figure 6. NB8 FAK shRNA cell motility on cFN is mediated by α4 and β1 but not by α5.

Figure 6

(a) Random motility tracks of NB8 cells expressing scrambled, FAK, p130Cas, or Src shRNA. Time-lapse images were collected every 4 min for 14 hrs and the scale is in μm. (b) Flow cytometry analyses of α4, α5, and β1 integrin expression (shaded peaks) in NB8 FAK shRNA cells. Open peaks show staining with control mAb. (c)* Haptotaxis motility of NB8 FAK shRNA expressing cells on cFN is in the presence of anti-α4 mAb (HP2/1, 10 μg/ml), anti-β1 mAb (P4C10, 10 μg/ml), and anti-α5 mAb (P1D6, 10 μg/ml). (d-e) Anti-p130Cas IPs from NB8 Scrambled or FAK shRNA cells (d) and Src shRNA cells (e) held in suspension (30 min) or plated on cFN (45 min) were analyzed by anti-phosphotyrosine (pY) followed by anti-p130Cas blotting. *See Fig.1.