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Yeast two-hybrid screen. An Arabidopsis cDNA prey library was screened using the WD40 region of 5PTase13 as a bait in the yeast two-hybrid system. Over 1 million yeast transformants were screened for reporter gene expression, which resulted in the isolation of a prey plasmid expressing the C-terminal domain of SnRK1.1. Serially diluted yeast strains were spotted onto agar plates that are either nonselective or selective for expression of the two-hybrid reporter genes.
