Figure 6.

SnRK1 activity in 5ptase13 mutants. SnRK1 activity was measured in crude plant extracts from 7-d-old seedlings precipitated with ammonium sulfate according to the method described (Radchuk et al., 2006) using an SPS peptide. Values for SnRK1 activity (nmol inorganic phosphate min⁻¹ mg⁻¹ protein) are normalized to WT1. Extracts are from seedlings grown in no nutrients (A), low nutrients (0.5× MS salts; B), or 6% Glc (0.5× MS salts, 6% Glc; C). Bars represent means ± se of three replicates. The experiment was independently repeated two times. *, P < 0.05 compared with the wild type.