Figure 9.

A, Expression of Glc- and ABA-inducible genes from dark-grown 4-d-old wild-type and 5ptase13 seedlings that were either untreated (control; 0.5× MS salts, 0.8% agar) or treated with 6% Glc (0.5× MS salts, 0.8% agar) in the dark for 3 d. The experiment was independently repeated two times. B, Verification of overexpression of 5PTase13 in complemented lines and a 5PTase13:GFP line. Total RNA (1–2 μg) was isolated, and semiquantitative RT-PCR was performed with the indicated primers (Supplemental Table S4). C, The 5PTase13:GFP gene complements the ABA-insensitive phenotype of 5ptase13-1 mutants. Seeds from WT1, 5ptase13-1, and 5ptase13-1 containing a 5PTase13:GFP transgene were germinated and grown in the light for 6 d on 0.5× MS salts, 0.8% agar, and 3 μm ABA. The germination rate was scored for each group of seeds at day 3. Values are means ± se (n = 50). The experiment was independently repeated two times. *, P < 0.05 compared with the 5ptase13-1 mutant.