Table III.
Quantification of silencing in VIGS experiments by real-time RT-PCR
| Plant | Gene Target | Leaf
|
Trichome
|
||
|---|---|---|---|---|---|
| Expression Ratioa | se | Expression Ratioa | se | ||
| N. benthamiana | BCKD E1-β | 0.12 | 0.03 | – | – |
| S. pennellii | BCKD E1-β | 0.37 | 0.12 | 0.24 | 0.04 |
| KAS IA | 0.14 | 0.02 | 0.30 | 0.03 | |
| KAS IB | 0.66 | 0.09 | 0.63 | 0.05 | |
Expression level in silenced tissue relative to the TRV2 control (n = 3).