Figure 2.

Experimental design for CATMA transcript profiling and distribution of MTX-responsive genes over the time course analysis. A, Schematic representation of the experimental procedure used for transcript profiling of the Arabidopsis cell response to MTX. Five comparisons (1–5) of MTX-treated cells and corresponding controls were performed at three time points (see “Materials and Methods” for details). B, Number of genes regulated in response to MTX treatment. Differentially expressed genes were selected by statistical analysis after Bonferroni correction (P-value cutoff of 0.05). Numbers in parenthesis correspond to highly regulated genes, i.e. genes for which the log₂(ratio) value was >1 or <−1 (2-fold changes).