Figure 4.

Measurements of folate pools in Arabidopsis cells treated with MTX alone or in combination with LV. Cells were grown in standard conditions or exposed to MTX (100 μm) alone or in combination with LV (5-formyl-THF, 500 μm). The following pools of folates were determined by LC-MS/MS (Zhang et al., 2005): 5-methyl-THF, 5-formyl-THF, THF plus 5,10-methylene-THF, 10-formyl-THF plus 5,10-methenyl-THF, and folic acid. Data are means of three biological replicates and sd. Values are available in Supplemental Table S1.