Figure 2.

Yeast two-hybrid assays. A, AtMTAN interacts with CBL3 in a vector-independent manner. B, Interaction specificity between AtMTAN and CBL3. The first panels at left show the arrangement of the Y190 yeast cells carrying the indicated pGBT and pGAD plasmids. The second and third panels display yeast growth on synthetic complete medium lacking Leu and Trp (SC-LT) and synthetic complete medium lacking His, Leu, and Trp (SC-HLW), respectively. The last panels show β-galactosidase activity (filter-lift assay). [See online article for color version of this figure.]