Figure 3.

Structural requirement of AtMTAN and CBL3 for their interaction. A, Deletion mutants of AtMTAN. Different regions of AtMTAN were cloned into the pGBT9.BS vector and transformed into Y190 yeast cells carrying pGAD.GH or pGAD.CBL3. B, Identification of the CBL3 region involved in the interaction with AtMTAN. A series of CBL3 deletion mutants were created in the pGBT9.BS vector and cotransformed into yeast cells with either pGAD.GH or pGAD.AtMTAN. Yeast growth on the selection medium (SC-HLW) was scored as growth (+) or no growth (−). Black boxes indicate the binding domain of the GAL4 transcription factor. Numbers in the white boxes indicate the beginning and the ending positions of each protein fragment.