Table I.
Top candidate genes differentially expressed between the wild-type and dor leaves under drought stress using ATH1 GeneChip
| Affy Probe Set ID | Description | AGI No. | dor-dr/ck Log2 Ratioa | P Valueb | Fold-Changec | Wild Type-dr/ck Log2 Ratioa | P Valueb | Fold-Changec |
|---|---|---|---|---|---|---|---|---|
| 257280_at | NCED3de | At3g14440 | 5.15 | 0.00002 | 35.5 | 2.2 | 0.000836 | 4.6 |
| 263570_at | AAO3e | At2g27150 | 3.05 | 0.000025 | 8.3 | 1.4 | 0.002744 | 2.6 |
| 247957_at | ABI2e | At5g57050 | 2.9 | 0.000194 | 7.5 | 1.75 | 0.000366 | 3.4 |
| 247095_at | RAB18d | At5g66400 | 6.1 | 0.00002 | 68.6 | 4.65 | 0.00002 | 25.1 |
| 248337_at | RD29Ad | At5g52310 | 5 | 0.00002 | 32.0 | 1 | 0.165514 | 2.0 |
| 248352_at | RD29B | At5g52300 | 6.5 | 0.00002 | 90.5 | 3.95 | 0.001028 | 15.5 |
| 252102_at | XERO2 | At3g50970 | 6.7 | 0.00002 | 104.0 | 3.05 | 0.00002 | 8.3 |
| 259231_at | AtPP2CA | At3g11410 | 2.85 | 0.00002 | 7.2 | 1.9 | 0.00002 | 3.7 |
| 259570_at | COR47 | At1g20440 | 4.65 | 0.00002 | 25.1 | 2.3 | 0.000025 | 4.9 |
| 262128_at | LEA | At1g52690 | 7.7 | 0.00002 | 207.9 | 4.7 | 0.000044 | 26.0 |
| 258347_at | LEA-like | At3g17520 | 10.35 | 0.00002 | 1,305.2 | 6.05 | 0.00002 | 66.3 |
| 265216_at | MAP kinase | At1g05100 | 3.9 | 0.00002 | 14.9 | 2.45 | 0.201916 | 5.5 |
| 264005_at | AGP | At2g22470 | 1.95 | 0.000229 | 3.9 | 0.5 | 0.631018 | 1.4 |
| 267080_at | Unknown | At2g41190 | 5.5 | 0.00002 | 45.3 | 4.4 | 0.00002 | 21.1 |
| 263881_at | Unknown | At2g21820 | 6.3 | 0.00002 | 78.8 | 5.7 | 0.00002 | 52.0 |
| 264524_at | tat-binding | At1g10070 | 4.95 | 2.15E-05 | 30.9 | 3.2 | 2.85E-05 | 9.2 |
Log2 ratio measures the change in expression level for each probe set between drought treatment (dr) versus control (ck). This change is expressed as the log2 ratio. A log2 ratio of 1 is the same as a fold-change of 2. Here, we averaged the log2 ratio for two biological replicates.
Change P value, which measures the probability that the expression levels of each probe set between drought treatment versus control are the same. Here, we averaged the change P value for two biological replicates.
Fold-change is calculated using the signal log ratio.
Indicates that the gene expression level was independently validated by qRT-PCR, shown in Supplemental Figure S7.
Indicates some key genes for ABA biosynthesis and signal transduction pathway, but was not shown in the Affymetrix 8k chip used previously (Leonhardt et al., 2004).