Table I.
Peroxisomal matrix proteins of higher plants, mammals, and yeasts containing the targeting signal PTS2 in the N terminus
TPTS2 is indicated in bold. The conserved Cys near the cleavage site for the peroxisomal processing peptidase in higher eukaryotes is indicated in bold and underlined. (Taken from Helm et al. [2007]. Copyright by The National Academy of Sciences of the USA.)
| Enzyme/Species | Plants and Mammals
|
||
|---|---|---|---|
| N-Terminal Presequence | Cleavage Site* | Mature Subunit | |
| Malate dehydrogenase | |||
| Watermelon | MQPIPDVNQRIARISAHLHPPKSQMEESSALRRANCR*-AKGGAPGFKVAI | ||
| Pumpkin | MKPIPDVNERIARISAHLPPKSQMEEGSVLRRANCR*-AKGGAPGFRVAI | ||
| Alfalfa | MEPNSYANSRITRIASHLNPPNLKMNEHGGSSLTNVHCR*-AKGGTPGFKVAI | ||
| Rice | MEDAAAAARRMERLASHLRPPASQMEESPLLRGSNCR*-AKGAAPGFKVAI | ||
| Rape | MPHKRIAMISAHLQPSFTPQMEAKNSVMGLESCR*-AKGGNPGFKVAI | ||
| Arabidopsis (At5g09660) | MEFRGDANQRIARISAHLTPQMEAKNSVIGRENCR*-AKGGNPGFKVAI | ||
| Arabidopsis (At2g22780) | MPDNQRIARISAHLNPPNLHNQIADGSGLNRVACR*-AKGGSPGFKVAI | ||
| Citrate synthase | |||
| Pumpkin | MPTDMELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCV*-SAQTMVAPP | ||
| Arabidopsis (At2g42790) | MEISERVRARLAVLSGHLSEGKQDSPAIERWCT*-SADTSVAPL | ||
| Arabidopsis (At3g58740) | MEISERARARLAVLNAHLTVSEPNQVLPAIEPWCT*-SAHITAAPH | ||
| Acyl-CoA oxidase | |||
| Pumpkin | MASPGEPNRTAEDESQAAARRIERLSLHLTPIPLDDSQGVEMETC*-AAGKAKA | ||
| Arabidopsis (At5g65110) | MESRREKNPMTEEESDGLIAARRIQRLSLHLSPSLTLSPSLPLVQTETC*-SAR | ||
| 3-Keto-acyl-CoA thiolase | |||
| Cucumber | MEKAINRQSILLHHLRPSSSAYTNESSLSASVC*-AAGDSASY | ||
| Pumpkin | MEKAINRQSILLHHLRPSSSAYSHESSLSASVC*-AAGDSASY | ||
| Mango | MEKAINRQSILLHHLRPSNSSSHNYESALAASVC*-AAGDSAAY | ||
| Rape | MEKAMERQRVLLEHLRPSSSSSHSFEGSLSASAC*-LAGDSAAY | ||
| Arabidopsis (At5g48880) | MEKAIERQRVLLEHLRPSSSSSHNYEASLSASAC*-LAGDSAAY | ||
| Rat | MSESVGRTSAMHRLQVVLGHLAGRPESSSALQAAPC*-SAGFPQAS | ||
| Human | MQRLQVVLGHLRGPADSGWMPQAAPC*-LSGAPQAS | ||
| Phytanoyl-CoA2-hydroxylase | |||
| Rat | MDYTRAGARLQVLLGHLGRPSALQIVAHPVSGPASPANFC*-PEQFQYTL | ||
| Alkyldihydroxyacetone phosphate synthase | |||
| Human | MAEAAAAAGGTGLGAGASYGSAADRDRDPDPDRAGRRLRVLSGHLLGRPREALSTNEC*-KARRA | ||
| Rat | MAEAAGEAGASERDPDAVRARRRLRVLSGHLLGRPQEAPSTNEC*-KARRAASA | ||
| Mouse | MAEAAAGEAGASERDPDAGRARRRLRVLSGHLLGRPQEAPSTNEC*-KARRAASA | ||
| Yeasts | |||
| 3-Keto-acyl-CoA thiolase | |||
| Saccharomyces cerevisiae | MSQRLQSIKDHLVLSAMGLGESKRKNSLLEK | ||
| Candida tropicalis | MDRLNQLSGQLKPNAKQSILQKNPDDVVIV | ||
| Yarrowia lypolytica | MDRLNNLATQLEQNPAKGLDAITSKNPDDV | ||
| Amine oxidase | |||
| H. polymorpha | MERLRQIASQATAASAAPARPAHPLDPLST | ||