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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2008 Nov 9;15(12):1278–1286. doi: 10.1038/nsmb.1512

Figure 2. Spastin-CHMP1B interactions.

Figure 2

(a) Yeast two-hybrid interactions between the spastin MIT domain bait and the indicated CHMP prey constructs were assayed using the HIS3 reporter (sequential 10-fold yeast dilutions shown). (b) Spastin MIT and CHMP1B baits were tested for yeast two-hybrid interactions as in (a) with the indicted CHMP1B prey constructs. (c) GST-fusion of the CHMP1B-CTR, but not CHMP1A-CTR, pulls down the spastin MIT domain in vitro. (d) SPR analysis of spastin and VPS4A MIT domain binding to immobilized CHMP1B-CTR. The analyte is either the spastin or VPS4A MIT domain, as indicated. (e) Tabulated binding constants (Kd), or their lower limits.